Difference between revisions of "Ketner Lab"

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(Antibodies)
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*Calculate MOI
 
*Calculate MOI
 
*Protein preps of infected 293 cells for westerns
 
*Protein preps of infected 293 cells for westerns
*[[Western Blots]]
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*[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot]
 
*P32 label DNA
 
*P32 label DNA
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*Quantitate number of virions in CsCl virus preps
  
 
==Materials==
 
==Materials==
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*[[anti-Late serum]]
 
*[[anti-Late serum]]
 
*[[anti-NANP hybridoma supernatant]]
 
*[[anti-NANP hybridoma supernatant]]
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===Plasmids===
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===Viruses===
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===Cells===
  
 
== Links ==
 
== Links ==

Latest revision as of 12:55, 20 April 2008

JHSPHbanner.jpg


Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blot
  • P32 label DNA
  • Quantitate number of virions in CsCl virus preps

Materials

Antibodies

Plasmids

Viruses

Cells

Links

Gary Ketner

JHSPH Magazine