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| ==Work Experience==
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| *Scientist, [http://www.archemix.com/ Archemix Corp.] (2006–??)
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| *Postdoc in the [http://mit.edu/sauerlab/ Sauer lab] at [http://web.mit.edu/ MIT] (2001–2006) | | *Postdoc in the [http://mit.edu/sauerlab/ Sauer lab] at [http://web.mit.edu/ MIT] (2001–2006) |
| **[[Sauer Lab|Sauer lab OWW page]] | | **[[Sauer Lab|Sauer lab OWW page]] |
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| ==Education==
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| *Ph.D. in Chemistry, [http://www.scripps.edu/e_index.html The Scripps Research Institute], 2001 (with [http://www.scripps.edu/mb/joyce/joycelab.html Gerald Joyce])
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| *B.S. in Chemistry, [http://www.umd.edu University of Maryland College Park], 1996 (with [http://biophysics.jhu.edu/woodson/index.html Sarah Woodson])
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| *B.S. in Psychology, University of Maryland College Park, 1996
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| == Science stuff I know something about... ==
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| *tmRNA (or SsrA/10Sa RNA for you purists)
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| *translation
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| *energy-dependent proteolysis in bacteria
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| *The RNA World
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| *molecular biology in a world without kits
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| *''in vitro'' selection and evolution of nucleic acids
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| *generation of nucleic acid and protein libraries
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| *''in vitro'' RNA techniques
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| *nucleic acid-protein interactions (''in vitro'' analysis and bacterial ''in vivo'' analysis)
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| *fishing experiments to look for things that interact with your RNA/DNA/protein of interest
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| *PCR (even colony PCR, although it is '''not''' a separate skill)
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| *bacterial protein expression/purification
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| *''in vivo'' assays with ''E. coli'' i.e. analyzing effects on proteins/RNA
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| *molecular cloning
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| *Western/Northern/Southern blot analysis
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| *oligonucleotide synthesis
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| *RNA/DNA sequencing ("old school" and "new school" techniques)
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| *chemical and enzymatic structural probing of RNA/DNA
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| *gels, all kinds of gels: agarose, native acrylamide, denaturing acrylamide, SDS-acrylamide, two-dimensional
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| *enzyme kinetics
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| *radiolabeling nucleic acids and proteins
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| *familiarity with some biophysical techniques (a bit of CD spectroscopy, a small amount of analytical ultracentrifugation)
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| *fluorescence spectroscopy (nothing too fancy: straight-up fluorescence and a bit of anisotropy)
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| ==Publications==
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| '''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16762842&query_hl=1&itool=pubmed_docsum Engineering controllable protein degradation.]'''
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| McGinness, K.E., Baker, T.A. & Sauer, R.T. (2006) Mol. Cell, 22, 701-707.
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| '''[http://www.pnas.org/cgi/content/full/101/37/13454 Ribosomal protein S1 binds mRNA and tmRNA similarly but plays distinct roles in translation of these molecules.]'''
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| McGinness, K.E. & Sauer, R.T. (2004) Proc. Natl. Acad. Sci. USA 101, 13454–13459.
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| '''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12677051&query_hl=1 A Glimpse into tmRNA-mediated Ribosome Rescue.]'''
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| Moore, S.D., McGinness, K.E. & Sauer, R.T. (2003) Science 300, 127–130. (comment)
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| '''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12573693&query_hl=1 In Search of an RNA Replicase Ribozyme.]'''
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| McGinness, K.E. & Joyce, G.F. (2003) Chem. Biol. 10, 5–14. (review)
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| '''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12031665&query_hl=1 Continuous In Vitro Evolution of a Ribozyme that Catalyzes Three Successive Nucleotidyl Addition Reactions.]'''
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| McGinness, K.E., Wright, M.C. & Joyce, G.F. (2002) Chem. Biol. 9, 585–596.
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| '''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11927255&query_hl=1 RNA-Catalyzed RNA Ligation on an External RNA Template.]'''
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| McGinness, K.E. & Joyce, G.F. (2002) Chem. Biol. 9, 297–307.
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| '''[http://www.jbc.org/cgi/content/full/277/4/2987 Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element.]'''
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| McGinness, K.E. & Joyce, G.F. (2002) J. Biol. Chem. 277, 2987–2991.
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| ==Contact==
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| If you're on [[Main Page|OWW]], post a message on my [[User talk:Kathmc|talk page]], or send me an [[Special:Emailuser/Kathmc|email]].<br>
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| If you're not on OWW, [[OpenWetWare: How to join|join now]]! <br>
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| ==Fun facts== | | ==Fun facts== |
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| *[[Special:Contributions/Kathmc|My OWW contributions.]]
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| *'''Who's visiting?''' (Thanks [[Barry Canton|Barry]]!) | | *'''Who's visiting?''' |
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| *Other former WFJL crew on OWW: [[Roshan Kumar]], [[User:Wmshih|William Shih]] | | *Here is an excellent tutorial on how [http://video.google.com/videoplay?docid=-2657697036715872139 translation] works, with an introduction by the 1971 version of nobel laureate [http://nobelprize.org/chemistry/laureates/1980/berg-cv.html Paul Berg]. I'd like to see it in classrooms everywhere. '''tRNA! peptide bond!''' |
| **Related former TSRI folk on OWW: [[Plachikkat Radha]]
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| ===Important things I've learned through real life experiences.===
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| *If you can't fix it with duct tape, a safety pin, or both, you should probably just throw it out.
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| *Almost everything is better with bacon.
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| *Never underestimate the importance of your [http://en.wikipedia.org/wiki/Karass karass] (although I like [http://oook.info/bloggery/karass.html this] definition better) and beware of [http://en.wikipedia.org/wiki/Granfalloon granfalloons].
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| *Very few people know who [http://en.wikipedia.org/wiki/Foghorn_Leghorn Foghorn Leghorn] is.
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| [[KM:Personal|<font size=4>"Personal"</font>]]
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