Difference between revisions of "Kathleen McGinness"

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[[KM:Personal|<font size=4>"Personal"</font>]]
[[KM:Personal|<font size=4>"Personal"</font>]]
I'm a fourth (at least!) generation Marylander who grew up in the happy Washington, D.C. suburb of Derwood. I began my college career majoring in Biology at [http://www.psu.edu/ Penn State], but after realizing that the bustling metropolis of State College was not for me, I transferred to the University of Maryland. When I started at Maryland, I realized that although I support the need for animal research, I don't have the stomach for it. So, I decided to change my major to Chemistry, and threw in a major in Psychology for good measure. While at Maryland, I had the opportunity to work in the Laboratory of Neurochemistry and the Laboratory of General and Comparative Biochemistry at [http://www.nimh.nih.gov/ NIMH] (yes, where the rats are from). I also got my first introduction into the RNA world by working in [http://biophysics.jhu.edu/faculty-pages/woodson.html Sarah Woodson's lab] while she was at Maryland. After four years (yes, that makes it a 5 year plan for college!), I packed up my worldly possessions and headed to sunny So. Cal. to do my graduate work at Scripps with Jerry Joyce, solidifying my love of the RNA world. Much like [[user:skosuri|Sr]]i, I fell in love with CA. So, after completing my Ph.D. in chemistry (biochemistry, really), I returned to the east coast to work at MIT. In Bob Sauer's lab I have expanded my science into the protein world, but I'm still an RNA girl at heart. When I get the time, I'll put up some of my current research here.
===Random Stuff===
*My new favorite website detailing an alternative theory to evolution ([http://www.venganza.org/ WWFSMD?]).
*Lab-related pet peeves (partial list...)
**beeping timers
**people from other labs that come in asking for something and don't introduce themselves first (is it so hard to say,"Hi, I'm <insert name here> from the <insert PI name here> lab"??)
*I have the messiest bench (and desk) in the lab. Paradoxically, I am a tyrant about cleanliness in common lab areas.
*[[KM's soapbox]]
[[Sauer Lab| Back to the Sauer lab]]
[[Sauer Lab| Back to the Sauer lab]]

Revision as of 15:28, 10 December 2005


Education/Work Experience

  • B.S. in Psychology, University of Maryland College Park, 1996

Science stuff I know something about...

  • tmRNA (or SsrA/10Sa RNA for you purists)
  • translation
  • energy-dependent proteolysis in bacteria
  • The RNA World
  • molecular biology in a world without kits
  • in vitro selection and evolution of nucleic acids
  • generation of nucleic acid and protein libraries
  • in vitro RNA techniques
  • nucleic acid-protein interactions
  • fishing experiments to look for things that interact with your RNA/DNA/protein of interest
  • PCR (even colony PCR, although it is not a separate skill)
  • bacterial protein expression/purification
  • in vivo assays with E. coli i.e. analyzing effects on proteins/RNA
  • molecular cloning
  • Western/Northern/Southern blot analysis
  • oligonucleotide synthesis
  • RNA/DNA sequencing
  • structural probing of RNA/DNA
  • gels, all kinds of gels: agarose, native acrylamide, denaturing acrylamide, SDS-acrylamide, two-dimensional
  • enzyme kinetics
  • radiolabeling nucleic acids and proteins


Ribosomal protein S1 binds mRNA and tmRNA similarly but plays distinct roles in translation of these molecules.

McGinness, K.E. & Sauer, R.T. (2004) Proc. Natl. Acad. Sci. USA 101, 13454–13459.

A Glimpse into tmRNA-mediated Ribosome Rescue.

Moore, S.D., McGinness, K.E. & Sauer, R.T. (2003) Science 300, 127–130. (comment)

In Search of an RNA Replicase Ribozyme.

McGinness, K.E. & Joyce, G.F. (2003) Chem. Biol. 10, 5–14. (review)

Continuous In Vitro Evolution of a Ribozyme that Catalyzes Three Successive Nucleotidyl Addition Reactions.

McGinness, K.E., Wright, M.C. & Joyce, G.F. (2002) Chem. Biol. 9, 585–596.

RNA-Catalyzed RNA Ligation on an External RNA Template.

McGinness, K.E. & Joyce, G.F. (2002) Chem. Biol. 9, 297–307.

Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element.

McGinness, K.E. & Joyce, G.F. (2002) J. Biol. Chem. 277, 2987–2991.


Back to the Sauer lab