Kafatos:Making 1kb+ Ladder Stock
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Making 1kb+ DNA Ladder Stock. We dilute the entire contents of the tube and make 5x 500uL aliquots
- 250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)
- 500uL 5x DNA Loading Buffer
- 1750uL AccuGene Water (Lonza)
- Add the 3 components above together
- Mix well and make 5 x 500uL aliquots
- Leave one working stock at 4C and keep the other frozen at -20C
- Use 10uL of this stock per lane (1ug of ladder per lane)
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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