Difference between revisions of "Jonesma Lab:Protocols:Chlorophyll"

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7) Spin down at 13k for 5min
 
7) Spin down at 13k for 5min
  
8) Dilute 1/10 with DMSO
+
8) Dilute 1/10 with DMSO (Final volume 200 μL)
  
 
9) Measure at 645nm, 663nm, 750nm
 
9) Measure at 645nm, 663nm, 750nm
  
10) Process results as described in Richardson et al.
+
10) Process results as described in Richardson et al. - Remember to subtract 750nm from other values

Latest revision as of 07:47, 22 April 2014

Chlorophyll extraction

Modified from Richardson et al. 2002

1) Pre-weigh collection tubes, with grinding beads

2) After tissue collection on N2, reweigh collection tubes

3) Pre-warm DMSO to 65°C in glass bottle

4) Grind samples on dry ice and spin down

5) Add 500μL warmed DMSO and vortex

6) Incubate at 65°C for 30min

7) Spin down at 13k for 5min

8) Dilute 1/10 with DMSO (Final volume 200 μL)

9) Measure at 645nm, 663nm, 750nm

10) Process results as described in Richardson et al. - Remember to subtract 750nm from other values