Difference between revisions of "Janet B. Matsen:Closed Lab Questions"

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*Q: The gel is dark where I loaded it -- what does this mean?  
 
*Q: The gel is dark where I loaded it -- what does this mean?  
 
**A: It probably means you overloaded the PCR with too many cells.
 
**A: It probably means you overloaded the PCR with too many cells.
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==My primers are yellow!==
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*Don't worry -- this is normal!  [[Image:Yellow_primers.jpg]]

Revision as of 21:27, 23 February 2012

Back to Janet

I define "closed" as "not bothering me incessantly." One can always learn more but we have to stop at a point otherwise we would never move forward with our research!

If you have any input/corrections/references please drop [User:Janet B. Matsen|me] a line.

Colony PCR

  • Q: The gel is dark where I loaded it -- what does this mean?
    • A: It probably means you overloaded the PCR with too many cells.

My primers are yellow!

  • Don't worry -- this is normal! Yellow primers.jpg