James P. McDonald Week 11

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Biological Terms

  1. Trehalose: "A crystalline disaccharide that is found in various organisms, is about half as sweet as sucrose, and is sometimes used as a sweetener in commercially prepared foods." [[1]]
  2. Batch Culture: "A large-scale closed system culture in which cells are grown in a fixed volume of nutrient culture medium under specific environmental conditions." [[2]]
  3. Transcriptome: "The complete set of RNA transcripts produced by the genome at any one time." [[3]]
  4. Ergosterol: "Crystalline steroid alcohol that occurs especially in yeast, molds, and ergot and is converted by ultraviolet irradiation ultimately into vitamin D2" [[4]]
  5. Cryostat: "An apparatus for maintaining a constant low temperature especially below 0°C" [[5]]
  6. Cuvette: "A transparent or translucent box-shaped container with precisely-measured dimensions for holding liquid samples to be put into a spectrophotometer." [[6]]
  7. Desaterase: "Any of several enzymes that putdouble bonds into the hydrocarbon areasof fatty acids." [[7]]
  8. Mannoproteins: "Yeast cell wall components that are proteins with large numbers of mannose groups attached; highly antigenic." [[8]]
  9. Prototrophic: "Strain's that have the same nutritional requirements as the wild-type strain." [[9]]
  10. Supernatant: "The soluble liquid fraction of a sample after centrifugation or precipitation of insoluble solids." [[10]]

Outline

Introduction

  • Transcriptional regulation was studied in Saccharomyces cerevisiae in response to low temperatures within a chemostat.
    • This study focused on long-term low-temperature acclimation rather than rapid transitions to low temperature or "cold shock."
    • Transcription levels of various genes were measured at low temperatures.
  • The main result of the study revealed that there are large differences in transcription levels in many of the genes between the literature data, that looked at for rapid transitions to cold temperatures, and this study, that looked at long-term low-temperature acclimation.

Significance

  • The effect of cold temperatures on transcription in yeast has been studied greatly.
    • The studies has always been done in batch cultures.
    • This study uses a steady-state chemostat model to eliminate the effects of specific growth rates.
  • Other studies focused on a rapid transition to low temperatures, a cold shock.
    • This study focuses looks at the effects on yeast transcription with a slow transition to low-temperatures.
    • This allowed the yeast to acclimate rather than quickly adapt.
  • The results show there are differences between this study and the literature data.

Methods

Results

Conclusion

Class Links

Journal Entries and Assignments

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