File:Homologous Recombination Deletion method one.png

From OpenWetWare
Revision as of 19:36, 10 February 2013 by Evan J. Weaver (talk | contribs)
Jump to: navigation, search
Original file(1,184 × 1,829 pixels, file size: 229 KB, MIME type: image/png)

Image Explanation

Linear sequences of DNA were created using PCR from synthetic primers (the red stretch of DNA). Regions A, B and C inside the target region inside the genome (green) and inside the linear PCR sequence (red) are homologous regions. Cm^R is antibiotic resistance, S indicates an I-ScsI cleavage site.

The DNA is electroporated into the cell, then a plasmid coding for recombinase elements used for recombination. Cells that succeed in recombination were isolated.

Another plasmid coding for I-SceI meganuclease was inserted into the cell to remove the Cm^R gene. Then, a version of Rec-A mediated DSB recombinational repair was used to ligate the ends together.

File history

Click on a date/time to view the file as it appeared at that time.

current19:33, 10 February 2013Thumbnail for version as of 19:33, 10 February 20131,184 × 1,829 (229 KB)Evan J. Weaver (talk | contribs)
  • You cannot overwrite this file.

The following page links to this file: