ISISBio:Internal/Lab Supplies: Difference between revisions

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The following to be ordered by Louise
The following to be ordered by Louise


 
* Rainbow protein marker (proved very popular)
* 1 kB DNA marker (Fisher BP2553-100)
* Bradford reagent or stuff to make it - have made enquiries. Should be ordered Fri 13th.
* Bradford reagent or stuff to make it - have made enquiries. Should be ordered Fri 13th.



Revision as of 07:24, 20 June 2008

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LAB SUPPLIES

Required items

The following to be ordered by Louise

  • Rainbow protein marker (proved very popular)
  • 1 kB DNA marker (Fisher BP2553-100)
  • Bradford reagent or stuff to make it - have made enquiries. Should be ordered Fri 13th.
  • A4 scanner with one button scanning - Cameron to sort out brand or issues
  • Jencons 412-167 spot labels
  • Furniture for Biolab and BAM room - in negotiation
  • Wire baskets
  • large tape dispenser for coloured tapes - getting VWR quote on a large number of things

Wish List

  • Benchtop centrifuge suitable for 50 mL Falcon tubes
  • Another 2 microcentrifuges one for the cold room, one for R80

Ordered items

Fri 20th June 2008

  • Fisher kilobase marker x2 one in stock one to follow
  • Fisher 25-500bp marker x2
  • Fisher Invitrogen Kanamycin sulphate 25g
  • NdeI and NaeI (Promega R7131 and R6801)
  • Merck dodecyl maltoside 25g


Thurs 12th June 2008

  • Bugbuster
  • Benzonase (from Novagen as well as bugbuster)
  • Glucose (if we don't have some but I don't think we do)

19th May 2008

  • Fisher by requisition fume hood and cabinet

Items arrived

  • Welded 2 tier trolley
  • Sterile pipettes 5ml
  • Sterile pipettes 10ml
  • Sterile pipettes 25ml
  • Sterile pipettes 50ml
  • Rhamnose (sugar - Fisher 17408-0250 for 25 g)
  • BioRad- Bio-Safe Coomassie Stain, 5L x1 A box with a tap on for easy dispensing
  • BioRad- Precision Plus Kaleidoscope Standards 500ul (50 applications at 10ul per well) x2 This is a rainbow marker which I used at Reading Uni and was brilliant because there is never a doubt about the size of each marker band. I'v ordered 2 so we can use one and when that is used up we re-order so we never run out. To be stored at -20 when not in use. Store at room temp or in cold room when in use.
  • Syringe filter sterile 0.2um 25mm
  • Syringe filter sterile 0.2um, 4mm
  • Vivaspin protien concentrators 0.1-0.5ml
  • Merck Dodecyl Maltoside 5g, overnight shipment requested
  • -80 freezer storage-fisher

Fri 6th June

Thurs 5th June

Weds 4th June

  • Ep tips box 0.1-20ul

Tues 3rd June

Mon 2nd June

Fri 30th May

Thurs 29th May

  • Fisher Revco racking x3

Weds 28th May

Tues 27th May

Mon 26th May

Fri 23rd May

BioRad Biosafe Coomassie Stain 5L

Thurs 22nd May

Weds 21st May

Tues 20th May

  • Novagen Rosetta2 pLyS (cat# 71401)
  • Promega KRX Competent cells (20 x 50 uL - cat# L3002)
  • Fisher 15ml tubes
  • Fisher 50ml tubes
  • Lab coats XXL light blue x3
  • 0.1ul-20ul tips in box x3
  • 100ul-1000ul tips in box x4
  • 5ml tips in box x2
  • 2ul-200ul tips in box x4

Mon 19th May

13 May

  • Ammonium Sulphate 2x500g
  • IPTG 2x10g
  • 4L flask x8
  • Face Shield sign x2
  • Face Shield x2
  • XL lab coat x6
  • XXL lab coat x3
  • HiTrap Desalting column 5 × 5 ml 17-1408-01 £112.00 GE life sciences
  • dodecyl maltoside 5g

arrived (ordered 1st May 2008)

  • BPE1422-2 Yeast Extract 2kg x1
  • BPE-310-500 HEPES Crystals 500g x1
  • BPE328-1 Sodium Hydrogen Carbonate x1
  • CRY-190-010Q Cryobox 10pk x2
  • FB50323 Weighing boat 5ml 1000pk x1
  • FB50325 Weighing boat 30ml 1000pk x1
  • FB50327 Weighing boat 100ml 1000pk x1
  • FB50377 Weighing boat 250ml 1000pk x1
  • CRY-595-030P Centrifuge tubes 35ml 10pk x2

arrived 22nd May 2008

  • 50 ml Falcon Tubes
  • 15 ml Falcon Tubes
  • Petri Dish Rack

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