IPTG

Isopropyl-beta-D-thiogalactopyranoside

Induces transcription from promoters regulated by LacI repressor.

Molecular weight is 238.31 g/mol. The chemical formula is here.

1. Dissolve 1g in 4196 μL deionized water to make 1M solution.
2. Filter sterilize with syringe and 0.22μm filter

Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:

• lacIq: cell strains which overproduce LacI repressor (ie. E. coli cell strain type D1210, aka. BBa_V1003)

For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG.