From OpenWetWare
Revision as of 14:31, 3 April 2008 by Austin J. Che (talk | contribs)
Jump to: navigation, search


Induces transcription from promoters regulated by LacI repressor.

Molecular weight is 238.31 g/mol. The chemical formula is here.

  1. Dissolve 1g in 4196 μL deionized water to make 1M solution.
  2. Filter sterilize with syringe and 0.22μm filter

Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:

For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG.