Difference between revisions of "IPTG"

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Isopropyl-beta-D-thiogalactopyranoside
 
Isopropyl-beta-D-thiogalactopyranoside
  
Induces transcription from promoters regulated by lacI repressor.
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Induces transcription from promoters regulated by LacI repressor.
  
 
Molecular weight is 238.31 g/mol.  The chemical formula is [http://www.fermentas.com/catalog/reagents/img/iptg.gif here].
 
Molecular weight is 238.31 g/mol.  The chemical formula is [http://www.fermentas.com/catalog/reagents/img/iptg.gif here].
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#Dissolve 1g in 4196 μL deionized water to make 1M solution.
 
#Dissolve 1g in 4196 μL deionized water to make 1M solution.
 
#Filter sterilize with syringe and 0.22μm filter
 
#Filter sterilize with syringe and 0.22μm filter
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Alternatively
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#Dissolve 238 mg IPTG (sigma I-6758) in 10 ml DW for (100mM stock)
 +
#store in 1 ml aliquotes at -20°C
  
 
Generally a 1mM solution is an effective amount to induce the pLac promoter region.  It should be noted that this may vary over cell strains.  For example:  
 
Generally a 1mM solution is an effective amount to induce the pLac promoter region.  It should be noted that this may vary over cell strains.  For example:  
*lacIq: cell strains which overproduce lacI repressor (ie. [[E. coli genotypes|E.coli cell strain]] type [[E. coli genotypes#D1210|D1210]], aka. [[registry:Part:BBa_V1003|BBa_V1003]])
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*lacIq: cell strains which overproduce LacI repressor (ie. [[E. coli genotypes|''E. coli'' cell strain]] type [[E. coli genotypes#D1210|D1210]], aka. [[registry:Part:BBa_V1003|BBa_V1003]])
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For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG.
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[[Category:Material]] [[Category:Chemical]]

Latest revision as of 13:26, 23 December 2011

Isopropyl-beta-D-thiogalactopyranoside

Induces transcription from promoters regulated by LacI repressor.

Molecular weight is 238.31 g/mol. The chemical formula is here.

  1. Dissolve 1g in 4196 μL deionized water to make 1M solution.
  2. Filter sterilize with syringe and 0.22μm filter

Alternatively

  1. Dissolve 238 mg IPTG (sigma I-6758) in 10 ml DW for (100mM stock)
  2. store in 1 ml aliquotes at -20°C

Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:

For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG.