IGEM:Virginia 2012/Protocols/SSM Media Preparation

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BASAL MEDIUM to make 1 Liter 2 Liters 4 Liters 5 Liters
Glutamic acid, monosodium salt, 1H2O 10.72g 21.44g 42.88g 53.60g
Trisma Base 1.525g 3.05g 6.10g 7.63g
NaCl 2.50g 5.00g 10.00g 12.50g
KH2PO4 0.50g 1.00g 2.00g 2.50g
KCl 0.20g 0.40g 0.80g 1.00g
MgCl2 6H2O 100mg 200mg 400mg 500mg
CaCl2 2H2O 20mg 40mg 80mg 100mg


  • Add 2.9g of NaCl to a 1 L bottle.
  • Add in 25 mL of 7.5 pH Tris-Cl
  • Bring this to a volume of 500 mL by adding distilled water.
  • Autoclave (large liquid).


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!



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