Difference between revisions of "IGEM:Virginia 2012/Protocols/PEG/NaCl Preparation"

From OpenWetWare
Jump to: navigation, search
(Procedure)
 
(One intermediate revision by the same user not shown)
Line 10: Line 10:
  
 
# Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
 
# Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add 20g four times or 40g twice).
+
# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add it in smaller increments).
 
# Measure 58.4g NaCl and add it slowly.
 
# Measure 58.4g NaCl and add it slowly.
 +
# Autoclave (large liquid).
 +
# Stir it on the magnetic mixer again.
  
 
==Notes==
 
==Notes==

Latest revision as of 10:09, 30 May 2012

Materials

  • 20% PEG-8000
  • 2.5 M NaCl
  • milliQ H2O
  • 1 L bottle
  • Stir bar

Procedure

  1. Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl.
  2. Measure out 80g of PEG-8000 and add it slowly (it might be easier to add it in smaller increments).
  3. Measure 58.4g NaCl and add it slowly.
  4. Autoclave (large liquid).
  5. Stir it on the magnetic mixer again.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!


References

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.