IGEM:Virginia 2012/Protocols

Here is a list of the protocols the 2012 Virginia iGEM team will be following in order to carry out our research project.

Protocols

• Phage Amplification
• Plaque Assay -- to quantify the amount of bacteriophage in a sample.
• Phage Isolation
• Phage Replication Rate Assay
• Preparing a Pertussis Culture
• Isolating the Phage Genome
• CaCl2 Competent Stock Cells
• Chemical transformation
• Using the NanoDrop

Solution & Plate Preparations

• Bordet Gengou (BG) Agar Plate Preparation for culturing B. pertussis.
• LB Broth or Agar Preparation for culturing B. bronchiseptica.
• PEG/NaCl Preparation
• SM Buffer Preparation
• SSM Media Preparation
• TE Buffer