Difference between revisions of "IGEM:Virginia 2012/Protocols"

From OpenWetWare
Jump to: navigation, search
(Solution & Plate Preparations)
m
 
(9 intermediate revisions by 3 users not shown)
Line 3: Line 3:
 
==Protocols==
 
==Protocols==
 
* [[/Phage Amplification|Phage Amplification]]
 
* [[/Phage Amplification|Phage Amplification]]
* [[/Plaque Assay|Plaque Assay]] -- to quantify the amount of bacteriophages in our samples.
+
* [[/Plaque Assay|Plaque Assay]] -- to quantify the amount of bacteriophage in a sample.
 
* [[/Phage Isolation|Phage Isolation]]
 
* [[/Phage Isolation|Phage Isolation]]
 
* [[/Phage Replication Rate Assay|Phage Replication Rate Assay]]
 
* [[/Phage Replication Rate Assay|Phage Replication Rate Assay]]
 
* [[/Preparing a Pertussis Culture|Preparing a Pertussis Culture]]
 
* [[/Preparing a Pertussis Culture|Preparing a Pertussis Culture]]
 +
* [[/Isolating the Phage Genome|Isolating the Phage Genome]]
 +
* [[/CaCl2 Competent Stock Cells | CaCl2 Competent Stock Cells]]
 +
* [http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf Chemical transformation]
 +
* [[/Using the NanoDrop | Using the NanoDrop]]
  
 
==Solution & Plate Preparations==
 
==Solution & Plate Preparations==
Line 14: Line 18:
 
* [[/SM Buffer Preparation|SM Buffer Preparation]]
 
* [[/SM Buffer Preparation|SM Buffer Preparation]]
 
* [[/SSM Media Preparation|SSM Media Preparation]]
 
* [[/SSM Media Preparation|SSM Media Preparation]]
 +
* [http://en.wikipedia.org/wiki/TE_buffer TE Buffer]

Latest revision as of 15:42, 2 October 2012

Here is a list of the protocols the 2012 Virginia iGEM team will be following in order to carry out our research project.

Protocols

Solution & Plate Preparations