Difference between revisions of "IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/10/23"

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• Due to the odd results of the last clearings, a 5 mL culture of BL21 was inoculated with material from the T7 and synCG plate.
 
• Due to the odd results of the last clearings, a 5 mL culture of BL21 was inoculated with material from the T7 and synCG plate.
 
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• Viral genome isolation was performed again with cell culture samples from each of the following: IDT (positive control); WildType T7 (negative control); T7 halves treated with CIAP; SynCG treated with CIAP; RBS and SynCG treated with CIAP; and RBS and SynCG not treated with CIAP.  However, upon performing PCR and gelling the products, no results formed.
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• Viral genome isolation was performed again with cell culture samples from each of the following: IDT plasmid (positive control); WildType T7 (negative control); T7 halves treated with CIAP; SynCG treated with CIAP; RBS and SynCG treated with CIAP; and RBS and SynCG not treated with CIAP.  We are currently validating results by detection PCR and pregnancy test kit.
  
  

Revision as of 18:46, 26 October 2012

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• The SynCG BioBrick was isolated again.
• Due to the odd results of the last clearings, a 5 mL culture of BL21 was inoculated with material from the T7 and synCG plate.
• Viral genome isolation was performed again with cell culture samples from each of the following: IDT plasmid (positive control); WildType T7 (negative control); T7 halves treated with CIAP; SynCG treated with CIAP; RBS and SynCG treated with CIAP; and RBS and SynCG not treated with CIAP. We are currently validating results by detection PCR and pregnancy test kit.