IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/10/03: Difference between revisions
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(Autocreate 2012/10/03 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough) |
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== | ==October 3rd== | ||
Overnight pregnancy test results (~17 hours after the bacteria was added): | |||
<br> | |||
Positive: | |||
<br> | |||
Sonication, combination of all cultures, no DDT | |||
<br> | |||
T7 lysis, combination of all cultures, no DDT | |||
<br> | |||
Negative: | |||
<br> | |||
Son., culture #2 only, no DTT | |||
<br> | |||
T7, #2, no DTT | |||
<br> | |||
Son., comb, no denaturing solution added | |||
<br> | |||
T7, comb, no den | |||
<br> | |||
Son., #2, no den | |||
<br> | |||
T7, #2, no den | |||
<br> | |||
The negative tests showed one clear control band and a light background color which extended 3/4 of the way across the window. The background color was slightly darker in the area where the positive band would normally appear. | |||
<br> | |||
The Western blot showed bands at the appropriate molecular weight for hCG in all 4 lanes of promotor + RBS + synCG | |||
Revision as of 17:29, 3 October 2012
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October 3rdOvernight pregnancy test results (~17 hours after the bacteria was added):
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