Difference between revisions of "IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/10/03"

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(Autocreate 2012/10/03 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough)
 
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Crunch Time</span>
 
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==Entry title==
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==October 3rd==
* Insert your content here.
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Overnight pregnancy test results (~17 hours after the bacteria was added):
 
+
<br>
 +
Positive:
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<br>
 +
Sonication, combination of all cultures, no DDT
 +
<br>
 +
T7 lysis, combination of all cultures, no DDT
 +
<br>
 +
Negative:
 +
<br>
 +
Son., culture #2 only, no DTT
 +
<br>
 +
T7, #2, no DTT
 +
<br>
 +
Son., comb, no denaturing solution added
 +
<br>
 +
T7, comb, no den
 +
<br>
 +
Son., #2, no den
 +
<br>
 +
T7, #2, no den
 +
<br>
 +
The negative tests showed one clear control band and a light background color which extended 3/4 of the way across the window. The background color was slightly darker in the area where the positive band would normally appear.
 +
<br>
 +
The Western blot showed bands at the appropriate molecular weight for hCG in all 4 lanes of promotor + RBS + synCG
  
  

Revision as of 16:29, 3 October 2012

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October 3rd

Overnight pregnancy test results (~17 hours after the bacteria was added):
Positive:
Sonication, combination of all cultures, no DDT
T7 lysis, combination of all cultures, no DDT
Negative:
Son., culture #2 only, no DTT
T7, #2, no DTT
Son., comb, no denaturing solution added
T7, comb, no den
Son., #2, no den
T7, #2, no den
The negative tests showed one clear control band and a light background color which extended 3/4 of the way across the window. The background color was slightly darker in the area where the positive band would normally appear.
The Western blot showed bands at the appropriate molecular weight for hCG in all 4 lanes of promotor + RBS + synCG