IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/09/30: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Week of 9/30/12</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Week of 9/30/12</span>
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Latest revision as of 22:05, 26 September 2017

Week of 9/30/12 Main project page
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Week of 9/30/12

Gel of the 12 PCR products showed no sign of the vector.
Repeated gel with P+RBS+SynCG plasmid #2 and plasmid #5, along with the 5 plasmid of P+RBS+SynCG from last week.
Performed pregnancy test experiments with SynCG biobricks in DH5α with two trials: one with only the #2 culture (the culture which the biobrick was extracted from) and one from the combined culture. These cultures were separated for sonication. Based off of previous results, no DTT will be added, only 8M Urea and no denaturing solution.



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