IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/08/05: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 11</span> | ||
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== | ==(8/5 – 8/11)== | ||
We prepped the Biobrick plasmid p581c3 | |||
<br> | |||
We got results for miniprep of the plasmid backbone. Based on the nanodrop results, | |||
the procedure did not work. We tried growing cultures in liquid. | |||
<br> | |||
The cultures grew up in liquid but not as well as on plates. We made glycerol stocks | |||
and did plasmid prep followed by detection PCR to confirm presence of inserts. | |||
<br> | |||
We found no plaques on the T7 plate so we were unsure if T7 got in to the cell. | |||
<br> | |||
Did research on the protocol for refolding hCG-beta protein. SDS-PAGE suggested | |||
that there was no expression of the desired fragments. | |||
<br> | |||
Carried out T7 phage amplification. | |||
<br> | |||
Ordered antibodies to use in western blot | |||
<br> | |||
Planned out how to assemble promotor + RBS + hCG-beta Biobrick + terminator on | |||
the biobrick-designated plasmid | |||
Revision as of 17:23, 3 October 2012
Week 11 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
(8/5 – 8/11)We prepped the Biobrick plasmid p581c3
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