Difference between revisions of "IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/08/05"

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(Autocreate 2012/08/05 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough)
 
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 11</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==Entry title==
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==(8/5 – 8/11)==
* Insert your content here.
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We prepped the Biobrick plasmid p581c3
 +
<br>
 +
We got results for miniprep of the plasmid backbone. Based on the nanodrop results,
 +
the procedure did not work. We tried growing cultures in liquid.
 +
<br>
 +
The cultures grew up in liquid but not as well as on plates. We made glycerol stocks
 +
and did plasmid prep followed by detection PCR to confirm presence of inserts.
 +
<br>
 +
We found no plaques on the T7 plate so we were unsure if T7 got in to the cell.
 +
<br>
 +
Did research on the protocol for refolding hCG-beta protein. SDS-PAGE suggested
 +
that there was no expression of the desired fragments.
 +
<br>
 +
Carried out T7 phage amplification.
 +
<br>
 +
Ordered antibodies to use in western blot
 +
<br>
 +
Planned out how to assemble promotor + RBS + hCG-beta Biobrick + terminator on
 +
the biobrick-designated plasmid
  
  

Revision as of 16:23, 3 October 2012

Igem-logo-150px.png Week 11 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

(8/5 – 8/11)

We prepped the Biobrick plasmid p581c3
We got results for miniprep of the plasmid backbone. Based on the nanodrop results, the procedure did not work. We tried growing cultures in liquid.
The cultures grew up in liquid but not as well as on plates. We made glycerol stocks and did plasmid prep followed by detection PCR to confirm presence of inserts.
We found no plaques on the T7 plate so we were unsure if T7 got in to the cell.
Did research on the protocol for refolding hCG-beta protein. SDS-PAGE suggested that there was no expression of the desired fragments.
Carried out T7 phage amplification.
Ordered antibodies to use in western blot
Planned out how to assemble promotor + RBS + hCG-beta Biobrick + terminator on the biobrick-designated plasmid