IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/07/01

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(7/1 – 7/7)

Attempted to find antibiotics to which RB53 was resistant to.
Performed plasmid miniprep on tube # 3,5,7,10 and then did nanodrop. The nanodrop values were very low, so we added a large volume of template DNA to the PCR reaction.
Did PCR clean up on hCG digest.
Performed nanodrop on cleaned up, CAIP-treated, and digested PET vector. The nanodrop, however, did not show any DNA present.