Difference between revisions of "IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/24"

From OpenWetWare
Jump to: navigation, search
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
 
{|{{table}} width="800"
 
{|{{table}} width="800"
 
|-
 
|-
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
+
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Week 5</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
==Entry title==
+
==(6/24 – 6/30)==
 
Performed Gibson Assembly on Luc genome and hCG genome. We then performed
 
Performed Gibson Assembly on Luc genome and hCG genome. We then performed
 
digest on the Gibson Assembly products. The results were hard to decipher.
 
digest on the Gibson Assembly products. The results were hard to decipher.

Latest revision as of 22:05, 26 September 2017

Igem-logo-150px.pngWeek 5 Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

(6/24 – 6/30)

Performed Gibson Assembly on Luc genome and hCG genome. We then performed digest on the Gibson Assembly products. The results were hard to decipher.
We tried various lysis techniques such as: BPER buffer, chloroform, and RIPA buffer.
Bought EPT and Clearblue pregnancy test kits. Their purpose was to detect for the presence of hCG. Unfortunately we got all negative results. We tried with E. coli first, but still got negative results meaning that it did not take up the hCG gene.
Tried additional sonication method for lysis.
Digest of phage genome and vector digest both worked. During PCR purification, the products were resuspended in too large a volume, thereby making them dilute.
The next step was to either concentrate them or run a larger volume of digested hCG plasmid in order to detect via traniluminator.