IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Week 5</span> | ||
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| colspan="2"| | | colspan="2"| | ||
== | ==(6/24 – 6/30)== | ||
Performed Gibson Assembly on Luc genome and hCG genome. We then performed | |||
digest on the Gibson Assembly products. The results were hard to decipher. | |||
<br> | |||
We tried various lysis techniques such as: BPER buffer, chloroform, and RIPA buffer. | |||
<br> | |||
Bought EPT and Clearblue pregnancy test kits. Their purpose was to detect for the | |||
presence of hCG. Unfortunately we got all negative results. We tried with E. coli first, | |||
but still got negative results meaning that it did not take up the hCG gene. | |||
<br> | |||
Tried additional sonication method for lysis. | |||
<br> | |||
Digest of phage genome and vector digest both worked. During PCR purification, | |||
the products were resuspended in too large a volume, thereby making them dilute. | |||
<br> | |||
The next step was to either concentrate them or run a larger volume of digested hCG | |||
plasmid in order to detect via traniluminator. | |||
Revision as of 17:17, 3 October 2012
Week 5 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
(6/24 – 6/30)Performed Gibson Assembly on Luc genome and hCG genome. We then performed
digest on the Gibson Assembly products. The results were hard to decipher.
|