IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/17: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 4</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==(6/17 – 6/23)== | ||
Attempted electrotransformation of Bordatella to electroporate DNA into the cells. | |||
This did not work. We found that RB50 and RB53 are not electrotransformable | |||
<br> | |||
Used Qiagen Viral RNA kit to purify phage genome and then did nanodrop | |||
successfully. Also performed gel electrophoresis to visualize size and quantity of | |||
DNA. | |||
<br> | |||
Performed PCR on isolated phage genome BPP | |||
<br> | |||
Gel electrophoresis image confirmed that the start and end of the BPP phage genome | |||
were what we thought they were. | |||
<br> | |||
The 260/280 ratio for nanodrop of the genomic phage DNA isolation was off. It | |||
needed to be closer to 1.8. | |||
<br> | |||
We attempted isolation of the hCG plasmid from DH5-alpha using Qiagen prep spin | |||
miniprep kit. | |||
Latest revision as of 22:05, 26 September 2017
Week 4 | Main project page Previous entry Next entry |
(6/17 – 6/23)Attempted electrotransformation of Bordatella to electroporate DNA into the cells.
This did not work. We found that RB50 and RB53 are not electrotransformable
|