Difference between revisions of "IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/11"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2012/06/11 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough)
 
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
 
{|{{table}} width="800"
 
{|{{table}} width="800"
 
|-
 
|-
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
+
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 3</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
==Entry title==
+
==(6/11 – 6-16)==
* Insert your content here.
+
Pipetted RB50 with plasmid and RB50 without plasmid on kanamycin plates and left
 +
them for incubation.
 +
<br>
 +
• We set up PCR for luciferase.
 +
<br>
 +
• Tried chemical transformation on RB50 with plasmid and without plasmid as well
 +
as DH5-alpha (as a positive control). We planned to transform with kanamycin
 +
resistance plasmid.
 +
<br>
 +
• Imaged gel from PCR of Luciferase/Kanamycin plasmids. It was successful.
 +
<br>
 +
• Used Qiagen PCR purification kit to clean up amplicons.
 +
<br>
 +
• Results of transformation: DH5-alpha grew. RB50 with and without plasmid,
 +
however, did not grow.
 +
<br>
 +
• Looking for ways to electroporate the DNA into the cells.
  
  

Latest revision as of 21:05, 26 September 2017

Igem-logo-150px.png Week 3 Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

(6/11 – 6-16)

Pipetted RB50 with plasmid and RB50 without plasmid on kanamycin plates and left them for incubation.
• We set up PCR for luciferase.
• Tried chemical transformation on RB50 with plasmid and without plasmid as well as DH5-alpha (as a positive control). We planned to transform with kanamycin resistance plasmid.
• Imaged gel from PCR of Luciferase/Kanamycin plasmids. It was successful.
• Used Qiagen PCR purification kit to clean up amplicons.
• Results of transformation: DH5-alpha grew. RB50 with and without plasmid, however, did not grow.
• Looking for ways to electroporate the DNA into the cells.