IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/11: Difference between revisions
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(Autocreate 2012/06/11 Entry for IGEM:Virginia/2012/Notebook/Genetically_engineered_bacteriophage_for_diagnosis_of_whooping_cough) |
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week 3</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==(6/11 – 6-16)== | ||
Pipetted RB50 with plasmid and RB50 without plasmid on kanamycin plates and left | |||
them for incubation. | |||
<br> | |||
• We set up PCR for luciferase. | |||
<br> | |||
• Tried chemical transformation on RB50 with plasmid and without plasmid as well | |||
as DH5-alpha (as a positive control). We planned to transform with kanamycin | |||
resistance plasmid. | |||
<br> | |||
• Imaged gel from PCR of Luciferase/Kanamycin plasmids. It was successful. | |||
<br> | |||
• Used Qiagen PCR purification kit to clean up amplicons. | |||
<br> | |||
• Results of transformation: DH5-alpha grew. RB50 with and without plasmid, | |||
however, did not grow. | |||
<br> | |||
• Looking for ways to electroporate the DNA into the cells. | |||
Revision as of 17:14, 3 October 2012
Week 3 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
(6/11 – 6-16)Pipetted RB50 with plasmid and RB50 without plasmid on kanamycin plates and left
them for incubation.
|