IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/04

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(6/4 – 6/9)

Attempted genome isolation. We also familiarized ourselves with the basics of the Nanodrop machine. We would check by quantifying the phage genome with Nanodrop. However we got a flat curve indicating that it did not work.
Sadly, chemical transformation did not work on Bordatella Bronchiseptica.
Performed gel extraction with gel a gel extraction kit along with primers Started 2 300mL cultures of Bordatella Bronchiseptica, added 20 uL phage for amplification and took time points. (morning and evening for 48 hours). We quantified with qPCR. Ran it twice (without diluting phage from each time point). PCR did not work on Bordatella plate
Tried to use a plaque assay on Bronchiseptica with differing amounts of phage to quantify the different time points.
Attempted freeze/thaw transformation in Bronchiseptica, which did not work.