Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/23"

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(Autocreate 2013/09/23 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
 
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==Entry title==
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==Floor Two=
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The three overnight cultures were retrieved from the incubator. Cultures three and four were used to inoculate four fresh 10ml LB liquids that contained Kanamycin and Chlorophenicol antibiotics. 100μL from each culture inoculated a fresh LB, resulting in two cultures per original culture. They were left in the shaking incubator at 37°C until OD600 reached 0.4-0.6.
 
 
  
  

Revision as of 04:19, 11 September 2013

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=Floor Two

The three overnight cultures were retrieved from the incubator. Cultures three and four were used to inoculate four fresh 10ml LB liquids that contained Kanamycin and Chlorophenicol antibiotics. 100μL from each culture inoculated a fresh LB, resulting in two cultures per original culture. They were left in the shaking incubator at 37°C until OD600 reached 0.4-0.6.