IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/15

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Floor Two

The two plates and control plate from the previous day were successful, so two colonies were picked from plate one and used to inoculate two 10ml LB solutions and left to incubate overnight at 37°C.
The sequencing data of the analysed biobricks were studied further. Sequence 1,2,5, and 6 all matched their expected DNA Sequences. Sequence 3 didn't match the expected sequence and sequence 4 contained extra NdeI restriction sites.
Two LB solutions were inoculated with BL21 PlySs and BL21 Rosetta PlySs seperately and incubated overnight at 37°C.

Fig.1:Transformation of pETAntA plasmid into BL21 E.Coli cells and spread onto LB agar + Kanamycin plates.