IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/15
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Further digestion reactions were performed on the samples from last week as well as controls (original DNA) with Nde1 or Xba1 as well as EcoR1 & Pst1.
(We were not supposed to use EcoR1 but there was no enough Xba1).
After incubation samples were run on a gel to analyse them.
All soil samples received so far were numbered and decanted into uniform tubes (1-38).
Samples 1-38 were diluted from 10^-1 up to 10^-10.
Soya flour media (SFM) was made in quantities of 6 x 500 ml - agar, soya flour, mannitol and tap water. Autoclaved for 1 hour then poured into plates to set.
Dilutions of 10^-2, 10^-4, 10^-6, 10^-8 and 10^-10 of samples 1-11 were plated on SFM + 500 ul in 500 ml of Nystatin (Ny) and Cyclohexamide (Cy).
Plates were incubated at 30° for 7 days.
Carried on e-mailing iGEm teams , but now focused on sediment after communications with Lab on floor 1.
Ben Thompson arrangements.
E-mailed iGEM contact (Nitwa ..) about the idea of an outreach registry, so that teams who are organizing a public engagement event (i.e. Forum) could have a infomrational resource pool to draw from, instead of starting from scrach each time.
Will follow up iGEM (Westminster) as they have a simliar project and may be interested in such a resource and collaborating in its construction.
Organized a meeting with Anne Osbourne (JIC) who spoke at this years SB6.0 SynBio conference. This is related to projects leading on from/inspired by iGEM and will focus on how iGEM bricks can be used in real research.