IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/10: Difference between revisions
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==Floor One== | |||
The results from the 16S sequences returned. The DNA sequences were run through BLAST to find matching sequences in the database ''table 1''. | |||
Table 1: The strains of samples 1, 5, 7, 8 and 10 found using BLAST analysis. | |||
[[Image:Graph.png]] | |||
==Floor Two== | |||
Colonies 2-4 from the plate transformed with BL21 cells containing pET28AntA and AntGRFP plasmids(16/08) were used to inoculate three fresh 10ml LB with added Kanamycin and Chlorophenicol. They were incubated overnight at 37°C with shaking. | |||
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==Floor Two== | ==Floor Two== | ||
Colonies 2-4 from the plate transformed with BL21 cells containing pET28AntA and AntGRFP plasmids(16/08) were used to inoculate three fresh 10ml LB with added Kanamycin and Chlorophenicol. They were incubated overnight at 37°C with shaking. | Colonies 2-4 from the plate transformed with BL21 cells containing pET28AntA and AntGRFP plasmids(16/08) were used to inoculate three fresh 10ml LB with added Kanamycin and Chlorophenicol. They were incubated overnight at 37°C with shaking. |
Revision as of 16:03, 29 September 2013
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Floor OneThe results from the 16S sequences returned. The DNA sequences were run through BLAST to find matching sequences in the database table 1. Table 1: The strains of samples 1, 5, 7, 8 and 10 found using BLAST analysis. Floor TwoColonies 2-4 from the plate transformed with BL21 cells containing pET28AntA and AntGRFP plasmids(16/08) were used to inoculate three fresh 10ml LB with added Kanamycin and Chlorophenicol. They were incubated overnight at 37°C with shaking.
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Floor Two
Colonies 2-4 from the plate transformed with BL21 cells containing pET28AntA and AntGRFP plasmids(16/08) were used to inoculate three fresh 10ml LB with added Kanamycin and Chlorophenicol. They were incubated overnight at 37°C with shaking.
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