Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/08/08"

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(Week 8 Day 4)
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* Picked colonies of ''Agrobacterium tumerfaciens'', 2, 3, 4, 10 and 11 and grew in an overnight culture with shaking with LB and ampicillin.
 
* Picked colonies of ''Agrobacterium tumerfaciens'', 2, 3, 4, 10 and 11 and grew in an overnight culture with shaking with LB and ampicillin.
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We invited the Cambridge iGEM team up to meet us and discuss each others projects as well as future collaborations and gave them a tour of the NRP, JIC and UEA.
  
  

Revision as of 12:20, 8 August 2014

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Week 8 Day 4

  • Spread the level 2 constructs A-G on spectinomycin plates with IPTG and XGAL for blue white selection as well as plating the transformation of vector pAGM8031.
  • Plated end linkers 2 and 3 on ampicillin
  • Picked colonies of Agrobacterium tumerfaciens, 2, 3, 4, 10 and 11 and grew in an overnight culture with shaking with LB and ampicillin.

We invited the Cambridge iGEM team up to meet us and discuss each others projects as well as future collaborations and gave them a tour of the NRP, JIC and UEA.