Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/08/08"

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(Autocreate 2014/08/08 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
 
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==Entry title==
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==Week 8 Day 4==
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* Spread the level 2 constructs A-G on spectinomycin plates with IPTG and XGAL for blue white selection as well as plating the transformation of vector pAGM8031.
  
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* Plated end linkers 2 and 3 on ampicillin
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* Picked colonies of ''Agrobacterium tumerfaciens'', 2, 3, 4, 10 and 11 and grew in an overnight culture with shaking with LB and ampicillin.
  
  
 
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Revision as of 12:12, 8 August 2014

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Week 8 Day 4

  • Spread the level 2 constructs A-G on spectinomycin plates with IPTG and XGAL for blue white selection as well as plating the transformation of vector pAGM8031.
  • Plated end linkers 2 and 3 on ampicillin
  • Picked colonies of Agrobacterium tumerfaciens, 2, 3, 4, 10 and 11 and grew in an overnight culture with shaking with LB and ampicillin.