Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/08/05"

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(Autocreate 2014/08/05 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
 
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==Entry title==
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==Week 8, Day 2==
* Insert your content here.
 
  
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Lab work
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- Added 2g of sucrose and 0.441g of MS salts to 100ml of sterile water.  Added Acetosyringone.
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- Spun down agrobacterium cultures grown on 4.8.14
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- Resuspended pellets in 10ml of prepared solution
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- Left to grow on the bench for 3 hours
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- Each construct infiltrated into one nicotiana benthamiana plant on two separate leaves
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- Mini preps of level 1 dig-ligs (1,5,9,15,16,17,18,19)
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- Quantification of DNA required for next level two dig-ligs
  
  

Revision as of 08:54, 6 August 2014

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Week 8, Day 2

Lab work


- Added 2g of sucrose and 0.441g of MS salts to 100ml of sterile water. Added Acetosyringone.

- Spun down agrobacterium cultures grown on 4.8.14

- Resuspended pellets in 10ml of prepared solution

- Left to grow on the bench for 3 hours

- Each construct infiltrated into one nicotiana benthamiana plant on two separate leaves


- Mini preps of level 1 dig-ligs (1,5,9,15,16,17,18,19)

- Quantification of DNA required for next level two dig-ligs