Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/12"

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
 
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Floor Two==
 
==Floor Two==
 
The plates from the previous day had colonies but they weren't red in colour. The BL21 cultures were retrieved and 5ml was used to prepare soluble and insoluble protein fractions. The other 5ml was used to prepare a miniprep of the DNA. Both samples were stored in the freezer at -20°C. <br>
 
The plates from the previous day had colonies but they weren't red in colour. The BL21 cultures were retrieved and 5ml was used to prepare soluble and insoluble protein fractions. The other 5ml was used to prepare a miniprep of the DNA. Both samples were stored in the freezer at -20°C. <br>
Colonies from plates containing Bba_J04450,  Bba_K1041000, Bba_K1041001 and BL21 [pET28AntA + AntgpRFP] were used to inoculate two cultures each with the relevant antibiotics. All cultures were left to incubate overnight at 37°C.
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An experiment was designed to measure the concentration of red the biobrick Bba_K1041000 compared to that of Bba_J04450. Colonies from plates containing Bba_J04450,  Bba_K1041000, Bba_K1041001 and BL21 [pET28AntA + AntgpRFP] were used to inoculate two cultures each with the relevant antibiotics. All cultures were left to incubate overnight at 37°C.
  
  

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Floor Two

The plates from the previous day had colonies but they weren't red in colour. The BL21 cultures were retrieved and 5ml was used to prepare soluble and insoluble protein fractions. The other 5ml was used to prepare a miniprep of the DNA. Both samples were stored in the freezer at -20°C.
An experiment was designed to measure the concentration of red the biobrick Bba_K1041000 compared to that of Bba_J04450. Colonies from plates containing Bba_J04450, Bba_K1041000, Bba_K1041001 and BL21 [pET28AntA + AntgpRFP] were used to inoculate two cultures each with the relevant antibiotics. All cultures were left to incubate overnight at 37°C.