Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/04"

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(Autocreate 2013/09/04 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
 
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==Entry title==
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==Floor One==
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Performed chromosomal DNA preps for 12 of the O/N cultures that appeared to lack contaminants and had large areas of clearance in the Bioassays.<br>
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The ligated vector (pMS82 with GUS insert) was transformed into chemically-competent <i>E. coli</i> cells through heat shock. The cells were then plated onto LB-NaCl (with 200 μL Hygromycin per 200 ml LB). There were 2 plates with 100 μL and 800 μL volumes plated respectively per ligation reaction.
  
  

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Floor One

Performed chromosomal DNA preps for 12 of the O/N cultures that appeared to lack contaminants and had large areas of clearance in the Bioassays.
The ligated vector (pMS82 with GUS insert) was transformed into chemically-competent E. coli cells through heat shock. The cells were then plated onto LB-NaCl (with 200 μL Hygromycin per 200 ml LB). There were 2 plates with 100 μL and 800 μL volumes plated respectively per ligation reaction.