IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/02
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The PCR of the new GUS gene was performed (8 x 20 ul reactions of same proportions as first time round).
Took photographs of all Bioassay plates where actinomycete colonies appeared to have a zone of clearing around them - indicating the production of an anti-fungal.
The plates that had been transformed with the ligation (J04450+neo) contained no colonies.