IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/30

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Floor One

Performed 38 more Candida albicans overlays of Bioassays. Made up 2 x 200 ml SNA - 200 ml distilled water, 1.6 g nutrient broth powder and 1 g agar. This was autoclaved and left too cool in 55°C water bath (removed before molten stage). A 200 ul volume of Candida was added to 5 ml SNA per plate before swirling to disperse.
Agarose gel electrophoresis was performed on the gradient PCR products (110 mV for 35 min). The GUS gene was visible in all reactions at approx. 1.8 Kb.
Filtered, concentrated and resuspended more spore stocks in glycerol.
Performed a QIAquick gel extraction of gel to retrieve the new GUS gene.
Re-streaked the conjugations (S4, S4 △antA) onto SFM (+ Ny) with Thiostrepton.