IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/28

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Floor One

To gain an idea of what strains of Streptomyces are growing on the plates, liquid cultures of the purified streaked Streptomyces plates for 16S sequencing were prepared by transferring colonies to LB (-NaCl) broth + a spring to aerate the cultures.

Started preparing spore stocks by adding approx. 2 ml glycerol to lawn plates and using a cotton bud to shift the spores, adding more glycerol if required. The resulting liquid was then filtered using cotton wool in a 5 ml syringe. We spore stocked 32 lawns, 35 are remaining atm.

Liquid cultures were set up for E. coli ETpuz cells - LB broth was inoculated with cultures from pAU3-45 and pMS82 plates. The pAU3-45 cells had 10 ul Apr and the pMS82 cells were inoculated with 10 ul Hyg in LB -NaCl as Hygromycin is salt-intolerant.

500 ml SFM + 10 ml MgCl2???