IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/23

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Floor Two

Our focus of the experiments was to prepare more DNA of the confirmed biobricks and to reclone our fourth brick.
To prepare biobrick J04450+neo; plasmids 3b and AntGpneo were cut with restriction enzymes NdeI and PstI. The samples were ran on a agarose gel and the large fragment of 3b and small fragment of AntGpneo were excised and purified seperately.
DNA from 3a and BBa_J04450 were transformed into α-select gold competent cells and spread on LB agar plates with added chlorophenicol antibiotics and incubated overnight at 37°C.