IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/21
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Minipreps of BL21 containing pETAntA and AntGp+RFP plasmids were prepared using 5ml from each overnight culture and incubated with PstI restriction enzyme for analysis by gel electrophoresis. We also prepared the biobrick BBa_K1041000 (sample 3b2) by cutting with restriction enzyme NdeI and analysing on an agarose gel. The large fragment was cut from the gel to be purified. This process should remove the extra NdeI sites that were introduced to the sequence.
100μL from two of the overnight cultures was used to inoculate two fresh 10ml LB with added antibiotics Kanamycin and Chlorophenicol. They were incubated for 2 hours at 37°C and then one culture was induced with IPTG.
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