IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/21
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Minipreps were prepared using 5ml from each overnight culture and incubated with PstI restriction enzyme for analysis by gel electrophoresis. We also prepared the biobrick BBa_K1041000 by cutting with restriction enzyme NdeI and analysing on an agarose gel. The large fragment was cut from the gel to be purified. This process should remove the extra NdeI sites that were introduced to the sequence.