Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/20"

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(Floor Two)
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==Floor One==
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Made 6 x 500 ml SFM agar media, autoclaved for 1 hr and poured into plates.
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Isolated more single colonies that resembled Streptomyces from soil sample plates.
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==Floor Two==
 
==Floor Two==
 
We inoculated cultures of BL21 containing pETAntA and Antgp+RFP into 10mls LB with antibiotics Kanamycin and Chlorophenicol. Four cultures were grown overnight at 37°C.
 
We inoculated cultures of BL21 containing pETAntA and Antgp+RFP into 10mls LB with antibiotics Kanamycin and Chlorophenicol. Four cultures were grown overnight at 37°C.

Revision as of 07:02, 22 August 2013

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Floor One

Made 6 x 500 ml SFM agar media, autoclaved for 1 hr and poured into plates.

Isolated more single colonies that resembled Streptomyces from soil sample plates.


Floor Two

We inoculated cultures of BL21 containing pETAntA and Antgp+RFP into 10mls LB with antibiotics Kanamycin and Chlorophenicol. Four cultures were grown overnight at 37°C.