IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/16
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De-replicated single Streptomyces colony plates when it appeared that we had duplicates. This is spore stock the minimum amount of lawns.
Filtered, concentrated and resuspended spore stocks in 20 % glycerol.
The two overnight cultures, containing BL21 cells that had been transformed with Pet28AntA were retrieved. Culture 1a was used to make competent cells, while sample 1b was induced with IPTG and left to incubate at 25°C overnight. While the cells were growing, minipreps of BL21 PlySs and BL21 rosetta PlySs cultures were prepared and stored in the freezer.
A meeting was held to discuss the upcoming forum event such as posters and layout for the tables.