Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/09"

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(Floor two)
(Floor two)
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Another restriction digest of all the biobricks was prepared with different enzymes, to produce larger fragments. We used the enzymes PvuII and Xho1 to enable us to distinguish between the biobricks as the digests, when analysed using agarose gel electorphoresis,  should produce different size fragments for each plasmid.  
 
Another restriction digest of all the biobricks was prepared with different enzymes, to produce larger fragments. We used the enzymes PvuII and Xho1 to enable us to distinguish between the biobricks as the digests, when analysed using agarose gel electorphoresis,  should produce different size fragments for each plasmid.  
 
[[Image:BIORAD 2013-08-09 16hr 21min.jpg|thumb|Fig. Analysis of restriction digest of biobricks using enzymes PvuII and XhoI. Lanes 1,3,5,7,9 contain samples 3b2,1 colony 1,1 colony 2, 2 colony 1, 2 colony 2 respectively, which were cut with PvuII. Lanes 2,4,6,8,10 contain samples 3b2, 1 colony 1, 1 colony 2, 2 colony 1, 2 colony 2 respectively, which were cut with XhoI.]]
 
[[Image:BIORAD 2013-08-09 16hr 21min.jpg|thumb|Fig. Analysis of restriction digest of biobricks using enzymes PvuII and XhoI. Lanes 1,3,5,7,9 contain samples 3b2,1 colony 1,1 colony 2, 2 colony 1, 2 colony 2 respectively, which were cut with PvuII. Lanes 2,4,6,8,10 contain samples 3b2, 1 colony 1, 1 colony 2, 2 colony 1, 2 colony 2 respectively, which were cut with XhoI.]]
 
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[[Image:BIORAD 2013-08-09 16hr 23min.jpg|thumb|Fig. Analysis of restriction digest of biobricks using enzymes PvuII, XhoI, EcoRI and PstI. Lanes 1,3,5,7, contain samples 2b1,2b2,2c1,2c2 respectively, which were cut with PvuII. Lanes 2,4,6,8, contain samples 2b1,2b2,2c1,2c2 respectively, which were cut with XhoI. Lanes 10,11,12 contain sample 3c cut with EcoRI, PstI and No enzyme respectively]]
  
 
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Floor two

Another restriction digest of all the biobricks was prepared with different enzymes, to produce larger fragments. We used the enzymes PvuII and Xho1 to enable us to distinguish between the biobricks as the digests, when analysed using agarose gel electorphoresis, should produce different size fragments for each plasmid.

Fig. Analysis of restriction digest of biobricks using enzymes PvuII and XhoI. Lanes 1,3,5,7,9 contain samples 3b2,1 colony 1,1 colony 2, 2 colony 1, 2 colony 2 respectively, which were cut with PvuII. Lanes 2,4,6,8,10 contain samples 3b2, 1 colony 1, 1 colony 2, 2 colony 1, 2 colony 2 respectively, which were cut with XhoI.
Fig. Analysis of restriction digest of biobricks using enzymes PvuII, XhoI, EcoRI and PstI. Lanes 1,3,5,7, contain samples 2b1,2b2,2c1,2c2 respectively, which were cut with PvuII. Lanes 2,4,6,8, contain samples 2b1,2b2,2c1,2c2 respectively, which were cut with XhoI. Lanes 10,11,12 contain sample 3c cut with EcoRI, PstI and No enzyme respectively