IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/29
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Streaked lawns of single Streptomyces colonies that appeared to be sporulating and had large clear single colonies.
Re-streaked contaminated single Streptomyces colony plates.
Streaked from the plates in the conjugated Steptomyces trial that had grown on the kanamycin plates (?).
Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel fig 1. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight.