Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/25"

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==Floor Two==
 
==Floor Two==
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The two ligation samples from the previous day were analysed by gel electrophoresis alongside controls and the samples from the 22nd July.
 +
[[Image:BIORAD 2013-07-25 15hr 59min.JPG|thumb|Fig. Analysis of Ligation reactions by gel electrophoresis. Lane 1 and 2 (24/07 ligations), lanes 3 and 4 controls, lane 5 and 6 samples from 22nd July (2b and 2c).]]
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The membrane was retrieved from the shaker and washed with TBST and TBS solutions respectively for ten minutes. It was then incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result.
 
The membrane was retrieved from the shaker and washed with TBST and TBS solutions respectively for ten minutes. It was then incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result.
  

Revision as of 06:36, 31 July 2013

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Floor Two

The two ligation samples from the previous day were analysed by gel electrophoresis alongside controls and the samples from the 22nd July.

Fig. Analysis of Ligation reactions by gel electrophoresis. Lane 1 and 2 (24/07 ligations), lanes 3 and 4 controls, lane 5 and 6 samples from 22nd July (2b and 2c).

The membrane was retrieved from the shaker and washed with TBST and TBS solutions respectively for ten minutes. It was then incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result.