IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/25

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Floor Two

The two ligation samples from the previous day were analysed by gel electrophoresis alongside controls (2b2L and 3b1L) and the samples 2b and 2c from the 22nd July.

Fig. Analysis of Ligation reactions by gel electrophoresis. Lane 1 and 2 contain samples 1 and 2, lanes 3 and 4 controls, lane 5 and 6 samples from 22nd July (2b and 2c)repectively

Transformation reactions were prepared using SOC media for the ligation reactions, J04450 plasmid and sample 3b from 11/07 (J04450 with Nde1 site).

The membrane was retrieved from the shaker and washed with TBST and TBS solutions respectively for ten minutes. It was then incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result.