Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/16"

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Seven</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Lab==
 
==Lab==
  
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===Floor Two===
 
More restriction digest reactions were performed, for all the samples as well as the original DNA as controls, using Pst1&Xba1 and Xba1&Nde1. After incubations samples were run on an agarose gel.
 
More restriction digest reactions were performed, for all the samples as well as the original DNA as controls, using Pst1&Xba1 and Xba1&Nde1. After incubations samples were run on an agarose gel.
  
 
In the meantime we prepared samples to send them for sequencing, by diluting the primers and checking the concentration of samples using the photometer.  
 
In the meantime we prepared samples to send them for sequencing, by diluting the primers and checking the concentration of samples using the photometer.  
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===Floor One===
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Another 6 x 500 ml of SFM was made, autoclaved and poured into plates to set.
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Dilutions of 10<sup>-4</sup>, 10<sup>-6</sup>, 10<sup>-8</sup> and 10<sup>-10</sup> were plated on SFM + Ny and Cy for samples 12-38.
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All plates were incubated at 30°C for 7 days.
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==Outreach==
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Preliminary results from Lab on floor 1 suggest we may have to slightly change the Forum outreach activity angle.
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Latest revision as of 22:04, 26 September 2017

Igem-logo-150px.png Week Seven Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Lab

Floor Two

More restriction digest reactions were performed, for all the samples as well as the original DNA as controls, using Pst1&Xba1 and Xba1&Nde1. After incubations samples were run on an agarose gel.

In the meantime we prepared samples to send them for sequencing, by diluting the primers and checking the concentration of samples using the photometer.

Floor One

Another 6 x 500 ml of SFM was made, autoclaved and poured into plates to set.

Dilutions of 10-4, 10-6, 10-8 and 10-10 were plated on SFM + Ny and Cy for samples 12-38.

All plates were incubated at 30°C for 7 days.

Outreach

Preliminary results from Lab on floor 1 suggest we may have to slightly change the Forum outreach activity angle.