Difference between revisions of "IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/11"

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[[Image:2013-07-11 cut sites confirmation.jpg|thumb|Fig. Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c  with Nde1 respectively.]]  
 
[[Image:2013-07-11 cut sites confirmation.jpg|thumb|Fig. Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c  with Nde1 respectively.]]  
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[[Image:FMHw4NaVv1qk6t2gg07NMBDmEMwdn6mFeHg2ZQIP6NU.jpg|thumb|jkbgkjhk]]
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The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.
 
The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.

Revision as of 09:52, 22 August 2013

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Lab

Cultures were retrieved from incubation and minipreps were performed following the protocol. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel to confirm the results.

Fig. Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c with Nde1 respectively.
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The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.

Outreach

As the UK meet up was the following day, we practiced and finalised our presentation.